USE OF WATER-SOLUBLE 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE FOR THE FLUORESCENT DETERMINATION OF URONIC-ACIDS AND CARBOXYLIC-ACIDS

被引：14
|
作者：
KOBAYASHI, M [1 ]
ICHISHIMA, E [1 ]
机构：
[1] TOHOKU UNIV,FAC AGR,DEPT AGR CHEM,SENDAI,MIYAGI 981,JAPAN
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 189卷 / 01期
关键词：
D O I：
10.1016/0003-2697(90)90056-F
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA method was effective in detecting not only acidic sugar but also carboxylic acid. Although the sensitivity of the EDC-OPA method was somewhat lower than that of amino acid determination by OPA, a very simple and convenient assay was attained for compounds containing a carboxyl group. © 1990.
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页码：122 / 125
页数：4
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