BIOASSAY OF BOVINE INTERLEUKIN-1-LIKE ACTIVITY

被引：9
|
作者：
WINSTANLEY, FP [1 ]
ECKERSALL, PD [1 ]
机构：
[1] UNIV GLASGOW, SCH VET, DEPT VET BIOCHEM, GLASGOW G61 1QH, SCOTLAND
来源：
RESEARCH IN VETERINARY SCIENCE | 1992年 / 52卷 / 03期
关键词：
D O I：
10.1016/0034-5288(92)90024-V
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A bioassay for bovine interleukin-1 (IL1) activity is described. The assay is based on the IL1-stimulated proliferation of a mouse T-lymphocyte cell line, D10(N4)M. Bovine mononuclear cells stimulated with lipopolysaccharide produce an interleukin-1-like activity which stimulated the growth of the D10(N4)M cell line in a dose-dependent manner. The stimulatory activity was neutralised by a combination of both anti-human IL1-alpha and anti-human IL1-beta sera. The quantity of IL1-like activity released from the mononuclear cells increased asymptotically with increasing lipopolysaccharide dose.
引用
收藏
页码：273 / 276
页数：4
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