EFFICIENT TRANSFORMATION OF THERMOTOLERANT METHANOL-UTILIZING STRAINS OF METHYLOPHILUS SPP VIA ELECTROPORATION

被引：0

作者：

HAIDER, MZ ^{[1
]}

ALTAHO, N ^{[1
]}

ALSALAMEEN, F ^{[1
]}

KADRI, MH ^{[1
]}

ALAMAD, S ^{[1
]}

SPANIER, E ^{[1
]}

机构：

[1] KUWAIT INST SCI RES,DEPT BIOTECHNOL,SAFAT 13109,KUWAIT

来源：

ACTA BIOTECHNOLOGICA | 1991年 / 11卷 / 04期

关键词：

D O I：

10.1002/abio.370110402

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plasmid transformation of three thermotolerant strains of methylotrophic bacterium Methylophilus spp. was investigated using three different methods. The plasmids could be transformed by electroporation and were stabily maintained in these methylotrophic bacteria. The calcium chloride (competent cell) method was successful only in the case of one of the three strains studied. Protoplast transformation of methylotrophic bacteria was not successful. The antibiotic resistance markers conferred resistance on the transformants, whereas the wild-type bacteria were sensitive to the antibiotics.

引用

页码：295 / 301

页数：7

共 18 条

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