ONLINE AND OFFLINE TOOLS: CRISPR/CAS OFF-TARGET DETECTION

Genome engineering field is a new revolution in biological research and to this new field several restriction endonucleases are available. Out of all these, based on easier construction, sensitivity and specificity Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) preferred by scientists for research and industrial purposes. However, off-target DNA cleavage causes limitation to researchers who are interested in using engineered nucleases in gene or cell therapy. To overcome this issue, there is a need to design gRNAs which will produce CRISPR/Cas products with high specificity. Till writing this manuscript, there is no compile details available on such bioinformatics tools. Therefore, in this review we are trying to provide platform of information for research scientist who are working on CRSPR-Cas technology so that they can use these online and offline tools to prevent CRISPR/Cas off-targeting in research and industrial field depending on the type of experiment.