DETECTION OF ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES (ANCA) USING THE IMMUNOPEROXIDASE METHOD

被引：0
|
作者：
INAMURA, K
FUKASE, S
OHTA, N
SAKATA, K
KOIKE, Y
机构：
来源：
ACTA OTO-LARYNGOLOGICA | 1994年
关键词：
ANCA; IMMUNOPEROXIDASE METHOD; WEGENERS GRANULOMATOSIS;
D O I：
暂无
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Anti-neutrophil cytoplasmic antibodies (ANCA), which are present in Wegener's granulomatosis (WG) and other systemic vasculitis, were detected using the immunoperoxidase method rather than the indirect immunofluorescence method. It was possible to distinguish ANCA patterns (c-ANCA, p-ANCA) by the immunoperoxidase method. When neutropil smears were treated with 0.4% hydrogen peroxide solution, the endogenous peroxidase activity was sufficiently inhibited. ANCA was also detected, using HL-60 cells (human promyelocytic leukemia) and THP-1 cells (human myelocytic leukemia) as substrates.
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页码：218 / 220
页数：3
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