STRUCTURE COMPARISON AND EVOLUTIONARY RELATIONS BETWEEN ELONGATION-FACTORS EF-TU (EF-1-ALPHA) AND SUP 2 PROTEINS

On the basis of high homology and structural similarity, three genes, SUP2 Saccharomyces cerevisiae, SUP2 Pichia pinus and GST1 Homo sapiens, might be considered as members of one family named SUP2. Comparison of the primary structure of SUP2 proteins and elongation factors EF-Tu(EF-1-alpha) from 19 different species was performed. It was found that SUP2 proteins bear more homology to eukaryotic elongation factor than to procaryotic EF-Tu, though the degree of sequence conservation in SUP2 proteins is smaller than in EF-1-alpha factors. The extensive phylogenetic analysis of SUP2 and EF-Tu(EF-1-alpha) genes was performed by means of 3 methods, 2 phenetic and one cladystic (maximal parsimony). The data support the close relation of SUP2 genes to other elongation factor genes.