Fluorescent dyes experiments were conducted to study host-feeding habits with wild collected, newly emerged first generation and laboratory reared domesticated the thirtieth generation adult Anopheles sinensis Wiedemann (An. sinensis) at Tangkou Township, Shandong Province, China. Of 5942 wild collected in the field, 10027 newly emerged first generation and 2761 domesticated the thirtieth generation adult An.. sinensis was released, according to the recaptured rate by human landing collections and cattle bait collections, showed that host-feeding habits of An. sinensis were not random, most wild collected An. sinensis were still tend to seek their original host, first generation An. sinensis were still had feeding habits as their parents do, the original host tropism weakened for the thirtieth generation of An. sinensis.