RAPID INDUCTION OF INTERCELLULAR-ADHESION MOLECULE-1 ON MONOCYTES AND MYELOMONOCYTIC CELL-LINES AFTER INTERFERON-GAMMA TREATMENT

The ICAM-1 molecule is an important adhesion factor that facilitates lymphocyte activation as well as the movement of lymphocytes into solid tissues. It is poorly expressed on circulating monocytes but higher levels have been described following the use of some activation factors. While previous work has emphasized the role of interferon gamma in inducing increased ICAM-1 expression on nonleukocytic cells, we have demonstrated time- and dose-dependent increases on human monocytes and two myelomonocytic cell lines (Rc2A & U937). The increased level of ICAM-1 expression on the Rc2A cells was associated with higher accessory cell activity as determined by an increased mitogen and allogeneic response but specific antibody inhibition studies indicated only a partial dependence (up to 50%) on this molecule. Class II MHC expression and IL-1 production were not elevated by IFNg treatment of these cells, indicating that other factors account for the remainder of the incremental activity observed following this treatment.