ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF VERTICILLIUM SPP USING ANTISERA PRODUCED TO V-DAHLIAE FROM POTATO

被引：17
|
作者：
SUNDARAM, S [1 ]
PLASENCIA, J [1 ]
BANTTARI, EE [1 ]
机构：
[1] UNIV MINNESOTA,DEPT PLANT PATHOL,ST PAUL,MN 55108
来源：
PHYTOPATHOLOGY | 1991年 / 81卷 / 12期
关键词：
EARLY DYING POTATOES; VERTICILLIUM WILT OF POTATOES;
D O I：
10.1094/Phyto-81-1485
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Polyclonal antisera were prepared against purified mycelial proteins from Verticillium dahliae, the predominant fungus species in the potato early dying complex. The antisera tested against crude mycelial preparations of Verticillium spp. using indirect enzyme-linked immunosorbent assay (ELISA) reacted positively with 11 of 12 V. dahliae isolates from potato, cotton, and soil, but negatively with one isolate from tomato. The antisera did not react with mycelial proteins from Fusarium spp. from potato and cotton, with a Colletotrichum sp. from potato, or with one isolate of Rhizoctonia solani from sugarbeet. Double antibody sandwich ELISA, using polyclonal antisera, detected V dahliae and V. albo-atrum in infected roots and stems of potato,
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页码：1485 / 1489
页数：5
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