CLONING THE CLOSTRIDIUM-THERMOCELLUM THERMOSTABLE LAMINARINASE GENE IN ESCHERICHIA-COLI - THE PROPERTIES OF THE ENZYME THUS PRODUCED

被引：21
|
作者：
ZVERLOV, VV
VELIKODVORSKAYA, GA
机构：
[1] Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, 123182
来源：
BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 11期
关键词：
D O I：
10.1007/BF01022600
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have cloned a 1.9-kb-long fragment of Clostridium thermocellum DNA which encodes laminarinase (EC 3.2.1.39). The enzyme hydrolyzes the β-1,3-glucoside bonds in β-1,3-and in mixed β-1,3-1,4-polyglucans. The enzyme's optimum pH value is around 8.5, temperature optimum -70°C. PAGE-determined mol. weight -32 kDa. © 1990 Kluwer Academic Publishers.
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页码：811 / 816
页数：6
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