COVALENT ATTACHMENT OF METHYLENE-BLUE TO OLIGONUCLEOTIDES

被引：9
|
作者：
SCHUBERT, F
KNAF, A
MOLLER, U
CECH, D
机构：
[1] Humboldt-Universität, Institut für Chemie, 10099 Berlin
来源：
NUCLEOSIDES & NUCLEOTIDES | 1995年 / 14卷 / 06期
关键词：
D O I：
10.1080/15257779508010703
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The synthesis and application of oligonucleotides derivatized by methylene blue are described. For that, a carboxylated methylene blue derivative was synthesized and transformed into an activated N-hydroxysuccinimidoester. The activated ester was reacted with 5'-aminoalkylated oligonucleotides. The labelled oligonucleotides were isolated and characterized both by reversed phase HPLC and MALDI mass spectrometry. Initial studies on analytical application of these oligonucleotide conjugates are discussed.
引用
收藏
页码：1437 / 1443
页数：7
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