ANALYSIS OF PHENPROCOUMON AND ITS HYDROXYLATED AND CONJUGATED METABOLITES IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION

被引：16
|
作者：
EDELBROEK, PM
VANKEMPEN, GMJ
HESSING, TJ
DEWOLFF, FA
机构：
[1] STATE UNIV LEIDEN HOSP,TOXICOL LAB,2333 AA LEIDEN,NETHERLANDS
[2] PSYCHIAT HOSP ENDEGEEST,BIOCHEM LAB,OEGSTGEEST,NETHERLANDS
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 02期
关键词：
D O I：
10.1016/S0378-4347(00)82337-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The anticoagulant phenprocoumon is mainly metabolized in humans to hydroxylated metabolites and their glucuronides. A method is described for the determination of phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon, 7-hydroxyphenprocoumon, and their glucuronide and sulphate conjugates in human urine. Reversed-phase high-performance liquid chromatography is performed after selective extraction with disposable quaternary amine columns of untreated, and β-glucuronidase- or sulphatase-treated urine samples. Urinary excretion data are presented for total, glucuronidated, sulphated and free phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon and 7-hydroxyphenprocoumon in twelve patients after an average daily dosage of 1.3-4.2 mg phenprocoumon. © 1990.
引用
收藏
页码：347 / 358
页数：12
相关论文
共 50 条
  • [1] DETERMINATION OF THE COUMARIN ANTICOAGULANT PHENPROCOUMON AND METABOLITES IN HUMAN PLASMA, URINE AND BREAST-MILK BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION
    DEVRIES, JX
    SCHMITZKUMMER, E
    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 655 (01): : 63 - 71
  • [2] DETERMINATION OF BENZENE METABOLITES IN URINE OF MICE BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    SCHAD, H
    SCHAFER, F
    WEBER, L
    SEIDEL, HJ
    JOURNAL OF CHROMATOGRAPHY, 1992, 593 (1-2): : 147 - 151
  • [3] SOLID-PHASE EXTRACTION OF PIRIBEDIL AND ITS METABOLITES FROM PLASMA AND URINE WITHOUT AND AFTER DECONJUGATION, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    SARATI, S
    CACCIA, S
    EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS, 1992, 17 (03) : 205 - 211
  • [4] DETERMINATION OF PENTOXIFYLLINE AND ITS METABOLITES IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH SOLID-PHASE EXTRACTION
    MANCINELLI, A
    PACE, S
    MARZO, A
    MARTELLI, EA
    PASSETTI, G
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 575 (01): : 101 - 107
  • [5] DETERMINATION OF TEMOZOLOMIDE IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION
    SHEN, F
    DECOSTERD, LA
    GANDER, M
    LEYVRAZ, S
    BIOLLAZ, J
    LEJEUNE, F
    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 667 (02): : 291 - 300
  • [6] SOLID-PHASE EXTRACTION OF OXCARBAZEPINE AND ITS METABOLITES FROM PLASMA FOR ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    HARTLEY, R
    GREEN, M
    LUCOCK, MD
    RYAN, S
    FORSYTHE, WI
    BIOMEDICAL CHROMATOGRAPHY, 1991, 5 (05) : 212 - 215
  • [7] DETERMINATION OF DEXTROMETHORPHAN AND DEXTRORPHAN IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION
    WENK, M
    TODESCO, L
    KELLER, B
    FOLLATH, F
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1991, 9 (04) : 341 - 344
  • [8] Bio-analysis of docetaxel and hydroxylated metabolites in human plasma by high-performance liquid chromatography and automated solid-phase extraction
    Rosing, H
    Lustig, V
    Koopman, FP
    Huinink, WWT
    Beijnen, JH
    JOURNAL OF CHROMATOGRAPHY B, 1997, 696 (01): : 89 - 98
  • [9] RAPID QUANTITATION OF METHOTREXATE AND ITS METABOLITES IN HUMAN-SERUM, URINE AND BILE, USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    NUERNBERG, B
    KOHLBRENNER, M
    FAULKNER, R
    FURST, DE
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 487 (02): : 476 - 482
  • [10] SIMULTANEOUS MEASUREMENT OF THE MAJOR METABOLITES OF DOLASETRON MESILATE IN HUMAN URINE USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    SANWALD, P
    HUEBERT, ND
    HAEGELE, KD
    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 661 (01): : 101 - 107
12345