INCREASE IN GALANIN AND NEUROPEPTIDE-Y MESSENGER-RNA IN LOCUS-CERULEUS FOLLOWING ACUTE RESERPINE TREATMENT

Galanin and neuropeptide Y are known to coexist with noradrenaline in neurons of the locus coeruleus. Furthermore, reserpine depletes noradrenaline from, and increases the synthesis and activity of the catecholamine- synthesizing enzyme tyrosine hydroxylase in, this nucleus. We have found by in situ hybridization of specific 35S-labelled oligonucleotide probes that a single dose of reserpine (10 mg/kg) induced a significant (70-145%) and long-lasting (up to at least 3 days) increase in the amount of galanin and neuropeptide Y mRNA in the locus coeruleus (along with the characteristic increase in tyrosine hydroxylase mRNA). These results suggest a similar reserpine-sensitive mechanism of galanin, neuropeptide Y and tyrosine hydroxylase gene regulation. © 1990.