ENHANCED SENSITIVITY OF A SANDWICH ELISA FOR THE DETECTION OF BRUCELLA-MELITENSIS IN MILK BY IMMUNOPRECIPITATION

被引：0
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作者：
HAMMER, P
GREISERWILKE, I
HAHN, G
机构：
来源：
KIELER MILCHWIRTSCHAFTLICHE FORSCHUNGSBERICHTE | 1992年 / 44卷 / 04期
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TS2 [食品工业];
学科分类号：
0832 ;
摘要：
An indirect double sandwich ELISA for detection of Brucella lipopolysaccharide antigen in milk was developed. The test used a combination of a monoclonal antibody coat for antigen capture and polyclonal antibodies for detection of bound antigen. It was specific for Brucella spp.. In order to increase the sensitivity of the test, the antigen was concentrated by immunoprecipitation after solubilization and removal of organic contaminants. After this treatment, the test detected 102 Brucella melitensis colony forming units (cfu) per ml of raw milk.
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页码：349 / 355
页数：7
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