PURIFICATION AND PROPERTIES OF PHOSPHOLIPASE-D FROM STREPTOMYCES-ANTIBIOTICUS

被引：39
|
作者：
SHIMBO, K
IWASAKI, Y
YAMANE, T
INA, K
机构：
[1] NAGOYA UNIV, FAC AGR, DEPT APPL BIOL SCI, CHIKUSA KU, NAGOYA 46101, JAPAN
[2] SHIZUOKA UNIV, FAC AGR, DEPT APPL BIOL CHEM, OHYA, SHIZUOKA 422, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期
关键词：
D O I：
10.1271/bbb.57.1946
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phospholipase D was purified from Streptomyces antibioticus by column chromatography and chromatofocusing. The enzyme preparation was electrophoretically homogeneous and the molecular weight of the enzyme was estimated to be 64,000. Its isoelectric point was around pH 6.5. The enzyme was most active at pH 5.5 and at around 60-degrees-C. It was stable between pH 4 and 8, and below 50-degrees-C.
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页码：1946 / 1948
页数：3
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