AMPEROMETRIC DETERMINATION OF CHOLINE AND ACETYLCHOLINE WITH ENZYMES IMMOBILIZED IN A PHOTOCROSS-LINKABLE POLYMER

被引：36
|
作者：
MARTY, JL
SODE, K
KARUBE, I
机构：
[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,4-6-1 KOMADA,MEGURO KU,TOKYO 153,JAPAN
[2] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,YOKOHAMA,KANAGAWA 227,JAPAN
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 228卷 / 01期
关键词：
D O I：
10.1016/S0003-2670(00)80477-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Choline and acetylcholine sensors were prepared by using choline oxidase and acetylcholinesterase, entrapped in photocross- linkable poly(vinyl alcohol) bearing styrylpyridinium (PVA-SbQ). The measurements were based on the detection of hydrogen peroxide liberated by an enzyme reaction (choline oxidase) or two sequential enzyme reactions (acetylcholine esterase and choline oxidase). The determination range for choline was 2.5-2-150 αmol 1-1 and for acetylcholine 20-2-750 αmol 1-1. The response times were 2-2-4 min. The immobilized enzyme membranes stored in a dry state were very stable and no loss of activity was observed after storage for 60 days. © 1990.
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页码：49 / 53
页数：5
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