THE PRODUCTION OF AZADIRACHTIN BY IN-VITRO TISSUE-CULTURES OF NEEM, AZADIRACHTA-INDICA

被引：27
|
作者：
ALLAN, EJ
EESWARA, JP
JOHNSON, S
MORDUE, AJ
MORGAN, ED
STUCHBURY, T
机构：
[1] UNIV ABERDEEN,DEPT AGR,ABERDEEN AB9 2TN,SCOTLAND
[2] UNIV KEELE,DEPT CHEM,KEELE ST5 5BG,STAFFS,ENGLAND
[3] UNIV ABERDEEN,DEPT ZOOL,ABERDEEN AB9 2TN,SCOTLAND
来源：
PESTICIDE SCIENCE | 1994年 / 42卷 / 03期
关键词：
D O I：
10.1002/ps.2780420302
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Callus produced from leaves of a Ghanaian strain of the neem tree, Azadirachta indica A. Juss has been shown to produce the natural insecticide azadirachtin when grown in a defined medium. The azadirachtin was isolated by standard procedures of solvent partition and column chromatography monitored by supercritical fluid chromatography. Biological activity was monitored with antifeedant tests using the desert locust (Schistocerca gregaria Forsk.). The azadirachtin was identified by chromatography on three independent chromatographic systems (SFC, HPLC and TLC) and two thin-layer colour tests. It has 100% antifeedant activity at < 0.04 mg litre(-1). The yield of azadirachtin was 0.0007% based on dry weight of callus.
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收藏
页码：147 / 152
页数：6
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