THE OCCURRENCE AND SOURCES OF NATURAL ANTIBODY IN HUMAN BILE AND SERUM AGAINST THE O-ANTIGENS OF 2 ESCHERICHIA-COLI SEROTYPES

Paired serum and bile samples from normal subjects as well as patients with biliary disease were tested for natural antibody to two individual Escherichia coli O antigens by ELISA. Serum antibody was most commonly of IgM and IgG class. Antibody was less frequently detected in bile and was more commonly IgM than IgA. with IgG activity detected infrequently. Little relation was apparent between antibody in paired samples: activity could be present in both serum and bile or in either fluid alone. Titres in paired samples also did not correspond when normalised with respect to the concentrations of relevant isotypes: bile was Frequently enriched for natural antibody as a proportion of total immunoglobulin compared with serum Secretory component‐bound antibody was detectable in some biles that contained IgA and/or IgM activity and in the serum of 33%. of subjects with biliary disorders but not in normal sera A series of paired samples taken from three individuals was also examined for antibody against each subject's own intestinal commensal E. coli. Serum IgM and IgG activity was present in all samples, but antibody in bile was less frequent and was of IgM or IgA class. These results suggest that natural antibody in human bile occurs independently of antibody in serum and that it is substantially derived from local sources. Copyright © 1990, Wiley Blackwell. All rights reserved