PEPTIDE-SYNTHESIS CATALYZED BY CYNARA-CARDUNCULUS L ACID PROTEASE IN AQUEOUS-ORGANIC BIPHASIC SYSTEMS

被引：4

作者：

BARROS, MT ^{[1
]}

FARO, CJ ^{[1
]}

PIRES, EMV ^{[1
]}

机构：

[1] UNIV COIMBRA,FAC CIENCIAS & TECNOL,DEPT BIOQUIM,P-3049 COIMBRA,PORTUGAL

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 06期

关键词：

D O I：

10.1007/BF00138558

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ability of Cynara cardunculus L. protease to perform peptide synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products were separated by RP-HPLC and identified by amino acid analysis and by EI-MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amount produced by pepsin under the same conditions.

引用

页码：653 / 656

页数：4

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