EFFECT OF INSULIN ON NA+,K+-ATPASE IN RAT COLLECTING DUCT

被引:50
|
作者
FERAILLE, E [1 ]
ROUSSELOT, M [1 ]
RAJERISON, R [1 ]
FAVRE, H [1 ]
机构
[1] COLL FRANCE, PHYSIOL CELLULAIRE LAB, CNRS, URA 219, F-75231 PARIS 05, FRANCE
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1995年 / 488卷 / 01期
关键词
D O I
10.1113/jphysiol.1995.sp020955
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. The collecting duct is involved in the whole antinatriuretic effect of insulin, as indicated in vitro by the stimulatory effect of the hormone on ouabain-sensitive Rb-86(+) uptake. Since Na+,K+-ATPase drives Na+ reabsorption, the contribution of the Na+ pump to the effect of insulin was investigated in rat isolated cortical and outer medullary collecting duct. 2. Insulin enhanced ouabain-sensitive Rb-86(+) uptake in the absence, as well as in the presence, of either 5 x 10(-4) M amiloride or 10(-3) M hydrochlorothiazide (HCT). Maximal ouabain-sensitive Rb-86(+) uptake, measured in Na+-loaded tubules, was also enhanced by insulin. The insulin effect persisted both in the absence of external Na+, when the Na+,K+-ATPase operates in a Rb+-Rb+ exchange mode, and in tubules depolarized by a high external concentration (20 mM) of Rb+ or by addition of 3 mM Ba2+. 3. Insulin treatment did not alter the intracellular Na and K concentrations, the specific binding of [H-3]ouabain measured in intact tubules, or the hydrolytic activity of Na+,K+ ATPase measured after permeabilization of the tubule cells. 4. In conclusion, in the rat collecting duct, insulin increased Na+,K+-ATPase-mediated cation transport independently of Na+ availability, membrane potential and recruitment of pump units. The effect of insulin was lost after cell permeabilization, suggesting the presence of a cytosolic factor which controls the turnover of Na+,K+-ATPase.
引用
收藏
页码:171 / 180
页数:10
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