DIFFERENTIAL ACTIVATION OF CYTOSOLIC PHOSPHOLIPASE A(2) (CPLA(2)) BY THROMBIN AND THROMBIN RECEPTOR AGONIST PEPTIDE IN HUMAN PLATELETS - EVIDENCE FOR ACTIVATION OF CPLA(2) INDEPENDENT OF THE MITOGEN-ACTIVATED PROTEIN-KINASES ERK1/2

The thrombin receptor agonist peptide SFLLRN was less effective than thrombin in eliciting the liberation of arachidonic acid and the generation of thromboxane A(2) by human platelets, We found that while SFLLRN evokes an initial transient increase in cytosolic free calcium concentration ([Ca2+](i)) of similar magnitude as that caused by thrombin, the SFLLRN-induced elevation of [Ca2+](i) declines more rapidly to near resting levels than that evoked by thrombin, suggesting that disparate levels of [Ca2+](i) may contribute to the attenuated arachidonic acid release. Furthermore, we observed that SFLLRN is less effective than thrombin in mediating the ''activating'' phosphorylation of cytosolic phospholipase A(2) (cPLA(2)). Both thrombin and SFLLRN rapidly and transiently activated kinases that phosphorylate the 21-residue synthetic peptide Thr(669) derived from the epidermal growth factor receptor, but the maximal activation of proline-directed kinases by SFLLRN was less pronounced than that by thrombin. MonoQ chromatography and immunoblot analysis of extracts from stimulated platelets revealed that while thrombin induced a prominent activation of the mitogen-activated protein kinases ERK1 and ERK2, SFLLRN completely failed to do so. On the other hand, SFLLRN, like thrombin, stimulated the activity of a proline-directed kinase distinct from ERK1/2, but the activation of this kinase was less pronounced following stimulation of platelets with SFLLRN compared with thrombin. We conclude 1) that the partial activation of cPLA(2) and the subsequent attenuated mobilization of arachidonic acid in response to SFLLRN may be the consequence of a less prolonged elevation of [Ca2+](i) and insufficient activation of proline-directed kinase(s) by SFLLRN and 2) that the ability of SFLLRN to mediate the activating phosphorylation of cPLA(2) in the absence of ERK1/2 stimulation suggests that, at least in human platelets, proline-directed kinases other than ERK1/2 may phosphorylate and activate cPLA(2).