ROLE OF TYROSINE M210 IN THE INITIAL CHARGE SEPARATION OF REACTION CENTERS OF RHODOBACTER-SPHAEROIDES

被引：103
|
作者：
FINKELE, U
LAUTERWASSER, C
ZINTH, W
GRAY, KA
OESTERHELT, D
机构：
[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
[2] TECH UNIV MUNICH,DEPT PHYS,W-8000 MUNICH 2,GERMANY
来源：
BIOCHEMISTRY | 1990年 / 29卷 / 37期
关键词：
D O I：
10.1021/bi00489a002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Femtosecond spectroscopy was used in combination with site-directed mutagenesis to study the influence of tyrosine M210 (YM210) on the primary electron transfer in the reaction center of Rhodobacter sphaeroides. The exchange of YM210 to phenylalanine caused the time constant of primary electron transfer to increase from 3.5 ± 0.4 ps to 16 ± 6 ps while the exchange to leucine increased the time constant even more to 22 ± 8 ps. The results suggest that tyrosine M210 is important for the fast rate of the primary electron transfer.© 1990, American Chemical Society. All rights reserved.
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收藏
页码：8517 / 8521
页数：5
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