Screening of polyphenolic compounds in Piper trioicum (Roxb.) extracts

Aims: Present study aims to reveal the polyphenol content in Piper trioicum (Roxb.) extracts. Materials and methods: In the present study, the phenolic content of the ethanol and water extracts of Piper trioicum (Roxb.) plants was determined by chromatography techniques like Thin layer chromatography, high performance thin layer Chromatography, high performance liquid chromatography and Gas chromatography with Mass spectrometric detection. Total phenol was determined by UV method. Results: All the results of analytical methods were compared with standard phenolic compounds. The ethanol extract contained maximum total phenolic content (2.323mg GAE/g) than the water extract. 13 compounds identified by chromatography techniques. The results suggested Arbutin, quercetin, caffeic acid, Ferulic acid, chlorogenic acid, Gallic acid and its derivatives may be present in ethanolic and water extracts of Piper trioicum. Conclusions: The water and ethanol could extract the highest concentration of polyphenols from the Piper trioicum (Roxb) plant; these extracts may have a good pharmacological potency due to the presence of polyphenols compounds.