FERTILE TRANSGENIC INDICA-RICE PLANTS OBTAINED BY ELECTROPORATION OF THE SEED EMBRYO CELLS

被引：1
|
作者：
XU, XP
LI, BJ
机构：
来源：
PLANT CELL REPORTS | 1994年 / 13卷 / 3-4期
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D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We have obtained fertile transgenic plants of Indica rice variety IR36, by using electroporation to transfer the neomycin phosphotransferase II (nptII) gene into cells of mature embryos. Resistant calli were selected in the presence of 30 mug/ml G418. Nearly thirty transgenic plants were regenerated within three months after transformation. Many of them yielded seeds following self-pollination. Data from molecular analysis and enzyme assay proved that the foreign gene was stably integrated into the genome of resistant calli, R0 and R1 plants, and also expressed. Mendelian segregation of the nptII gene was observed in R1 progeny plants.
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页码：237 / 242
页数：6
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