QUANTIFICATION BY IMAGE-ANALYSIS OF IMMUNOCYTOCHEMICAL REACTIONS - APPLICATION FOR DETERMINATION OF LYSOZYME CONTENT IN INDIVIDUAL SMEARED CELLS

被引：17
|
作者：
CHAMPELOVIER, P
SEIGNEURIN, D
CHRISTOPHE, P
KOLODIE, L
机构：
[1] CHU GRENOBLE,HEMATOL LAB,F-38043 GRENOBLE,FRANCE
[2] FAC MED GRENOBLE,HISTOL LAB,EQUIPE MICROSCOPIE QUANT,F-38700 LA TRONCHE,FRANCE
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1991年 / 39卷 / 01期
关键词：
D O I：
10.1177/39.1.1701187
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The objective of the present study was to develop a cytophotometric technique to quantitate immunocytochemical reactions. Cell antigens were detected after immunophosphatase alkaline staining procedure. The amount of reaction product was quantitated by computerized scanning cytophotometry. The technical conditions (dilution of primary antibody; incubation time of the three antibodies; volume and pH of the enzyme substrate reaction; storage of the slides) required for optimal cytophotometric determination of the reaction product were determined. Under these optimally defined conditions, a linear relationship between cell protein content (lysozyme) and microdensitometric measure of the colored reaction product was found. This method could be used for other cells, antigens, and enzymatic indicators.
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页码：31 / 36
页数：6
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