CLONING AND CHARACTERIZATION OF AN AMINOPEPTIDASE P-ENCODING GENE FROM STREPTOMYCES-LIVIDANS

被引：28
|
作者：
BUTLER, MJ [1 ]
BERGERON, A [1 ]
SOOSTMEYER, G [1 ]
ZIMNY, T [1 ]
MALEK, LT [1 ]
机构：
[1] CANGENE CORP,MISSISSAUGA L4V 1T4,ON,CANADA
来源：
GENE | 1993年 / 123卷 / 01期
关键词：
INTRACELLULAR PEPTIDASE; EXPRESSION SCREENING; CHROMOGENIC PEPTIDE SUBSTRATES;
D O I：
10.1016/0378-1119(93)90549-I
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
An aminopeptidase P(PepP)-encoding gene has been cloned from Streptomyces lividans 66 by screening for overexpression of activity using the chromogenic substrate Gly-Pro-beta-naphthylamide as a liquid overlayer on colonies growing on agar medium. The pepP gene was localised by deletion mapping, and the nucleotide sequence was determined. The deduced amino acid sequence was found to display significant similarity to Escherichia coli PepP. The partially purified S.lividans enzyme had a 50-kDa subunit and was present as a homodimer. Direct Edman degradation of the purified protein confirmed that pepP encoded the observed intracellular PepP.
引用
收藏
页码：115 / 119
页数：5
相关论文
共 50 条
  • [1] CLONING AND CHARACTERIZATION OF A GENE ENCODING EXTRACELLULAR METALLOPROTEASE FROM STREPTOMYCES-LIVIDANS
    LICHENSTEIN, HS
    BUSSE, LA
    SMITH, GA
    NARHI, LO
    MCGINLEY, MO
    ROHDE, MF
    KATZOWITZ, JL
    ZUKOWSKI, MM
    GENE, 1992, 111 (01) : 125 - 130
  • [2] CLONING OF THE XYLANASE GENE OF STREPTOMYCES-LIVIDANS
    MONDOU, F
    SHARECK, F
    MOROSOLI, R
    KLUEPFEL, D
    GENE, 1986, 49 (03) : 323 - 329
  • [3] CLONING AND CHARACTERIZATION OF A GENE ENCODING A SECRETED TRIPEPTIDYL AMINOPEPTIDASE FROM STREPTOMYCES-LIVIDANS-66
    BUTLER, MJ
    BINNIE, C
    DIZONNO, MA
    KRYGSMAN, P
    SOLTES, GA
    SOOSTMEYER, G
    WALCZYK, E
    MALEK, LT
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (08) : 3145 - 3150
  • [4] CLONING AND CHARACTERIZATION OF AN ALPHA-AMYLASE GENE FROM STREPTOMYCES-LIVIDANS
    TSAO, LS
    LIN, LL
    CHEN, JC
    CHEN, JH
    HSU, WH
    BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1171 (03) : 255 - 262
  • [5] CLONING AND EXPRESSION OF THE TYROSINASE GENE FROM STREPTOMYCES-ANTIBIOTICUS IN STREPTOMYCES-LIVIDANS
    KATZ, E
    THOMPSON, CJ
    HOPWOOD, DA
    JOURNAL OF GENERAL MICROBIOLOGY, 1983, 129 (SEP): : 2703 - 2714
  • [6] CLONING, CHARACTERIZATION, AND EXPRESSION IN STREPTOMYCES-LIVIDANS 66 OF AN EXTRACELLULAR LIPASE-ENCODING GENE FROM STREPTOMYCES SP M11
    PEREZ, C
    JUAREZ, K
    GARCIACASTELLS, E
    SOBERON, G
    SERVINGONZALEZ, L
    GENE, 1993, 123 (01) : 109 - 114
  • [7] CLONING OF A 2ND XYLANASE-ENCODING GENE OF STREPTOMYCES-LIVIDANS 66
    VATSMEHTA, S
    BOUVRETTE, P
    SHARECK, F
    MOROSOLI, R
    KLUEPFEL, D
    GENE, 1990, 86 (01) : 119 - 122
  • [8] CLONING AND AMPLIFIED EXPRESSION IN STREPTOMYCES-LIVIDANS OF THE GENE ENCODING THE EXTRACELLULAR BETA-LACTAMASE FROM STREPTOMYCES-CACAOI
    LENZINI, MV
    NOJIMA, S
    DUSART, J
    OGAWARA, H
    DEHOTTAY, P
    FRERE, JM
    GHUYSEN, JM
    JOURNAL OF GENERAL MICROBIOLOGY, 1987, 133 : 2915 - 2920
  • [9] CLONING, EXPRESSION, AND CHARACTERIZATION OF THE MICROMONOSPORA-VIRIDIFACIENS NEURAMINIDASE GENE IN STREPTOMYCES-LIVIDANS
    SAKURADA, K
    OHTA, T
    HASEGAWA, M
    JOURNAL OF BACTERIOLOGY, 1992, 174 (21) : 6896 - 6903
  • [10] CLONING OF TYROSINASE GENE FROM STREPTOMYCES-LIVIDANS IN ESCHERICHIA-COLI
    NAYAK, KK
    PANDEY, NK
    ROY, P
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1988, 25 (06): : 515 - 517
12345