PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIIDIOTYPIC ANTIBODIES TO MURAMYLPEPTIDE

被引：2
|
作者：
MAREEVA, TY [1 ]
KOTOVA, OV [1 ]
GOLOVINA, TN [1 ]
NESMEYANOV, VA [1 ]
机构：
[1] RUSSIAN ACAD SCI, SHEMYAKIN OVCHINNIKOV INST BIORGAN CHEM, MOSCOW 117871, RUSSIA
来源：
FEBS LETTERS | 1994年 / 356卷 / 01期
关键词：
ANTIIDIOTYPIC ANTIBODY; MONOCLONAL ANTIBODY; MURAMYL PEPTIDE;
D O I：
10.1016/0014-5793(94)01220-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hybridomas producing monoclonal anti-idiotypic antibodies (anti-id MAbs) to N-acetylglucosaminyl-beta 1-4-N-acetylmuramyl-alanyl-D-isoglutamine (GMDP) were developed. Three clones of hybridomas demonstrated the properties characteristic for the Ab2 beta type of anti-id antibody: they bound to Fab-fragments of high-affinity MAb to GMDP; dose-dependent inhibition of this binding by GMDP was observed; immunization of mice with these MAbs resulted in production of GMDP-specific antibodies. When these antibodies were used to stain blots from SDS-PAGE of macrophage lysate, the same receptor proteins were specifically stained as upon staining with I-125-labelled GMDP derivative.
引用
收藏
页码：13 / 16
页数：4
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