REUSABLE NEURONAL BIOSENSOR FOR THE DETERMINATION OF 3-ACETYL PYRIDINE NEUROTOXIN

Development of a reusable neuronal sensor selective to the neurotoxin 3-acetyl pyridine is reported. The antennular structure from fresh water crayfish (Procamburus clarkii) was used as a sensing element to measure the frequency of action potential responses evoked by chemical stimulation of nerve cells. Reusability of the sensor was studied with emphasis on the reproducibility of action potential responses. Characterization of the novel sensor in terms of selectivity, response time and dose/response relationship is reported. Current status of lifetime extension and future directions of neuronal biosensor research are discussed.