Study of Bacteria isolated from Urinary Tract Infections and to Determine their Susceptibility for Antibiotics at a Teaching Hospital

Aim: To determine the pathogens causing urinary tract infections and susceptibility to antibiotics. Methods: In this study, 300 patients with clinical symptoms and suspected UTI were sampled. Using the method of Clean-Catch, urine samples were collected from patients admitted to the Medical Ward at Aziz Fatima Hospital, Faisalabad from 1st January 2012 until 31st December 2012. Samples were sent to the Laboratory Diagnostics and Research laboratory of Pathology Aziz Fatima Hospital, Faisalabad. Urine specimens were cultured for detection of microbial agents of UTI. A total of 230 urine culture sensitivity reports were analyzed. The bacteria isolated were recognized by means of biochemical tests. Results: In this study, 230 of 300 were shown to be positive for urine culture. The predominant single bacterial growth was observed in 200 (87%). The most frequent isolated organism was E. coli (82%). The other bacteria were Klebsiella sp. (10.5%), Pseudomonas sp. (4.5%), and Staphylococcus aureus (3%). Gram negative bacteria were more sensitive to Amikacin (85.41%) and Nitrofurantoin (80.15%). The isolated gram positive cocci were sensitive to Ceftazidime (88.8%), Nitrofurantoin (83.3%), Norfloxacin (77.7%) and Piperacillin-tazobactam (77.7%). Conclusion: Gram-negative bacilli were accountable for the urinary tract infections in our patients. E. coli. was most frequent bacteria isolated and the most efficient antimicrobial agents were Amikacin, Nitrofurantoin, Meropenem and Cefipime in contrary to gram-negative bacilli and Nitrofurantoin, Ceftazidime and Norfloxacin in opposition to gram positive cocci.