DEVELOPMENT AND COMPARISON OF PURIFICATION STRATEGIES FOR CHICKEN ANTIBODIES FROM EGG-YOLK

被引：0

作者：

SVENDSEN, L ^{[1
]}

CROWLEY, A ^{[1
]}

OSTERGAARD, LH ^{[1
]}

STODULSKI, G ^{[1
]}

HAU, J ^{[1
]}

机构：

[1] AARHUS UNIV,DEPT CHEM,DK-8000 AARHUS C,DENMARK

来源：

LABORATORY ANIMAL SCIENCE | 1995年 / 45卷 / 01期

关键词：

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

With regard for animal welfare as well as cost, antibodies purified from the egg yolk of immunized chickens are an attractive alternative to antibodies raised in serum of mammals. Various purification strategies based on initial purification by a freeze and-thaw method followed by one of three precipitation methods-ammonium sulfate, polyethylene glycol 6000, or caprylic acid-were compared, Ammonium sulfate precipitation (25 to 40%) was found to give the best combination of high purity and yield (58%). Moreover, this method is inexpensive and well suited for standard purification of egg yolk antibodies in large scale production facilities.

引用

页码：89 / 93

页数：5

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