SUPPRESSION OF CORNEAL GRAFT-REJECTION BY SUBCONJUNCTIVAL INJECTION OF FK-506 IN A RAT MODEL OF PENETRATING KERATOPLASTY

被引：1
|
作者：
MINAMOTO, A
SAKATA, H
OKADA, K
FUJIHARA, M
机构：
[1] HIROSHIMA RED CROSS HOSP,DEPT PATHOL,HIROSHIMA,JAPAN
[2] ATOM BOMB SURVIVORS HOSP,HIROSHIMA,JAPAN
来源：
JAPANESE JOURNAL OF OPHTHALMOLOGY | 1995年 / 39卷 / 01期
关键词：
CYTOTOXIC T LYMPHOCYTE; FK-506; GRAFT REJECTION; PENETRATING KERATOPLASTY; RAT;
D O I：
暂无
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
The usefulness of the subconjunctival injection of FK-506 for suppression of allograft rejection was investigated in a rat model of orthotopic penetrating keratoplasty. Fischer rats were used as donors and Dark Agouti rats, as recipients. FK-506 was administered subconjunctivally in a dosage of 0.3 mg/kg/day for 15 consecutive days after penetrating keratoplasty was performed. Allograft rejection occurred within 8 to 10 days after keratoplasty in all untreated rats (n=6). None of the FK-506-treated rats (n=6) exhibited graft rejection during the 3-week observation period. Histologic examination showed marked infiltration of mononuclear cells in the stroma of corneal grafts from untreated rats 3 weeks after grafting. Inflammatory cells were only occasionally observed in grafts from FK-506-treated rats, Donor-specific cytotoxic T lymphocyte activity was completely suppressed in FK-506-treated rats 3 weeks after grafting. Our results indicated that subconjunctival injection of FK-506 effectively prevented corneal allograft rejection in a rat model of penetrating keratoplasty.
引用
收藏
页码：12 / 19
页数：8
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