A MICROTITER PLATE-BASED HIGH-THROUGHPUT DNA PURIFICATION METHOD

被引：18
|
作者：
WANG, K
GAN, L
BOYSEN, C
HOOD, L
机构：
[1] Department of Molecular Biotechnology, University of Washington, Seattle, WA 98195
来源：
ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期
关键词：
D O I：
10.1006/abio.1995.1195
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96-well membrane-bottomed assay plate. With this method, 50% of the DNA is recovered routinely using Sephacryl-500HR as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations. (C) 1995 Academic Press, Inc.
引用
收藏
页码：85 / 90
页数：6
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