INHIBITION OF PNEUMOCYSTIS-CARINII DIHYDROPTEROATE SYNTHETASE BY SULFA DRUGS

被引：17
|
作者：
MERALI, S [1 ]
ZHANG, Y [1 ]
SLOAN, D [1 ]
MESHNICK, S [1 ]
机构：
[1] CUNY CITY COLL,DEPT MICROBIOL,NEW YORK,NY 10031
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1990年 / 34卷 / 06期
关键词：
D O I：
10.1128/AAC.34.6.1075
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 40 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.
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页码：1075 / 1078
页数：4
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