A NOVEL METHOD FOR IMMOBILIZATION OF PROTEOLYTIC-ENZYMES IN POLYMER HYDROGELS

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作者：
MARKVICHEVA, EA
BRONIN, AS
KUDRYAVTSEVA, NE
KUZKINA, IF
PASHKIN, II
KIRSH, YE
RUMSH, LD
ZUBOV, VP
机构：
[1] YA OVCHINNIKOV BIOORGAN CHEM INST,MOSCOW,RUSSIA
[2] MV LOMONOSOV FINE CHEM TECHNOL INST,MOSCOW,RUSSIA
[3] LY KARPOV PHYS CHEM RES INST,MOSCOW,RUSSIA
来源：
BIOORGANICHESKAYA KHIMIYA | 1994年 / 20卷 / 03期
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摘要：
A novel one-step method for enzyme immobilization in composite gels based on the thermally reversible polymer poly(N-vinylcaprolactam) was developed. Conditions for entrapment of trypsin and carboxypeptidase B in the gels were optimized. After immobilization, 80 and 90% of the original carboxypeptidase B and trypsin activities, respectively, were found to retain. Both immobilizes enzymes were active in a wider pH and temperature range. The granules with entrapped enzymes were successfully applied to obtain human insulin from recombinant proinsulin.
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页码：257 / 262
页数：6
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