SYNERGY BETWEEN ZINC AND PHORBOL ESTER IN TRANSLOCATION OF PROTEIN-KINASE-C TO CYTOSKELETON

被引:83
|
作者
ZALEWSKI, PD [1 ]
FORBES, IJ [1 ]
GIANNAKIS, C [1 ]
COWLED, PA [1 ]
BETTS, WH [1 ]
机构
[1] QUEEN ELIZABETH HOSP,RHEUMATOL UNIT,WOODVILLE,SA 5011,AUSTRALIA
关键词
Cytoskeleton; Phorbol ester; Protein kinase C; Zinc ionophore;
D O I
10.1016/0014-5793(90)81067-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinase C was measured in the cytoskeletal fraction of lymphocytes, platelets and HL60 cells, by specific binding of [3H]phorbol dibutyrate and by immunoblotting with antibody to a consensus sequence in the regulatory domain of α-, β- and γ-isozymes of protein kinase C. Treatment of cells for 40 min with a combination of zinc (2-50 μM), zinc ionophore pyrithione and unlabelled phorbol dibutyrate (200 nM) caused up to a ten-fold increase in cytoskeletal protein kinase C and a corresponding decrease in other cellular compartments. Omission of any of the reagents resulted in much less or no translocation. These effects were inhibited by 1,10-phenanthroline, which chelates zinc, and were not seen with calcium. Increase in cytoskeletal protein kinase C persisted for several hours and appeared to involve attachment of the enzyme to actin microfilaments. We propose that zinc, like calcium, regulates the distribution of PKC in cells. However, unlike calcium which controls the binding of PKC to the lipid component on cell membranes, zinc controls the distribution of PKC to membrane cytoskeleton, possibly actin. © 1990.
引用
收藏
页码:131 / 134
页数:4
相关论文
共 50 条
  • [1] PROTEIN-KINASE-C NEGATIVELY MODULATED BY PHORBOL ESTER
    INAGAKI, M
    HAGIWARA, M
    SAITOH, M
    HIDAKA, H
    [J]. FEBS LETTERS, 1986, 202 (02) : 277 - 281
  • [2] PHOSPHORYLATION OF THE PHORBOL ESTER RECEPTOR PROTEIN-KINASE-C
    ASHENDEL, CL
    HERMSEN, L
    MINOR, PL
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1986, : 191 - 191
  • [3] PHORBOL ESTER AND DIACYLGLYCEROL TRANSLOCATION OF PROTEIN-KINASE-C ACTIVITY IN CULTURED COLLECTING TUBULAR EPITHELIUM
    DIXON, BS
    BURKE, C
    BRECKON, R
    ANDERSON, RJ
    [J]. KIDNEY INTERNATIONAL, 1987, 31 (01) : 164 - 164
  • [4] SYNERGY BETWEEN PHORBOL ESTERS AND RETINOIC ACID IN INDUCING PROTEIN-KINASE-C ACTIVATION
    BOUZINBASEGARD, H
    FAN, XT
    PERDERISET, M
    CASTAGNA, M
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 204 (01) : 112 - 119
  • [5] PROTEIN-KINASE-C AS THE RECEPTOR FOR THE PHORBOL ESTER TUMOR PROMOTERS
    BLUMBERG, PM
    [J]. PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1987, 28 : 466 - 466
  • [6] PHORBOL ESTER INDUCED DEGRADATION OF IMMUNOPRECIPITABLE PROTEIN-KINASE-C
    BALLESTER, R
    ROSEN, OM
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1986, : 126 - 126
  • [7] CHARACTERIZATION OF PHORBOL ESTER BINDING TO PROTEIN-KINASE-C ISOTYPES
    DIMITRIJEVIC, SM
    RYVES, WJ
    PARKER, PJ
    EVANS, FJ
    [J]. MOLECULAR PHARMACOLOGY, 1995, 48 (02) : 259 - 267
  • [8] DOWN-REGULATION OF PROTEIN-KINASE-C BY PHORBOL ESTER
    INAGAKI, M
    HAGIWARA, M
    SAITOH, M
    HIDAKA, H
    [J]. JAPANESE JOURNAL OF PHARMACOLOGY, 1986, 40 : P105 - P105
  • [9] DIFFERENT TRANSLOCATION AND CALCIUM DEPENDENCE OF PROTEIN-KINASE-C ISOFORMS INDUCED BY PHORBOL ESTER IN HUMAN PLATELETS
    CRABOS, M
    FABBRO, D
    IMBER, R
    WOODTLI, T
    ERNE, P
    [J]. FASEB JOURNAL, 1991, 5 (04): : A898 - A898
  • [10] PROTEIN-KINASE-C, PHORBOL ESTERS AND MESSENGER-RNA TRANSLOCATION
    AITKEN, SJM
    BRYSON, BA
    AGUTTER, PS
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1987, 15 (03) : 412 - 413