SPECIFIC BINDING OF ATP TO EXTRACELLULAR SITES ON TORPEDO ACETYLCHOLINE-RECEPTOR

被引:14
|
作者
CARLSON, BJ
RAFTERY, MA
机构
[1] UNIV MINNESOTA,DEPT BIOCHEM,ST PAUL,MN 55108
[2] UNIV MINNESOTA,DEPT PHARMACOL,MINNEAPOLIS,MN 55455
关键词
D O I
10.1021/bi00080a002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-P-32]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with increasing concentration of 8-azido-ATP and the inhibition of photolabeling by ATP. Protection studies suggest that the binding sites for the photolabel are unique and are not associated with the cholinergic ligand binding sites.
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页码:7329 / 7333
页数:5
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