ASSIGNMENT OF DISULFIDE BONDS IN THE X-PROTEIN (HBX) OF HEPATITIS-B VIRUS

被引：25
|
作者：
GUPTA, A [1 ]
MAL, TK [1 ]
JAYASURYAN, N [1 ]
CHAUHAN, VS [1 ]
机构：
[1] INT CTR GENET ENGN & BIOTECHNOL,NEW DELHI 110067,INDIA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.2057
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have established the disulphide arrangement of cysteines in E. coli expressed HBx by chromatographic analysis of enzymatically cleaved protein and sequence analysis of cysteine containing fragments. Eight of the nine cysteines are disulphide linked in an interesting pattern. Each cysteine is linked to the fourth cysteine in a sequential manner and the last cysteine is free; the disulphide linkages are between Cys(7) and Cys(78), CyS(17) and CyS(115), Cys(61) and Cys(137), Cys(69) and CyS(143) while Cys(148) is free. (C) 1995 Academic Press, Inc.
引用
收藏
页码：919 / 924
页数：6
相关论文
共 50 条
  • [1] THE X-PROTEIN OF HEPATITIS-B VIRUS - FACTS AND FICTION
    WILL, H
    [J]. JOURNAL OF HEPATOLOGY, 1991, 13 : S56 - S57
  • [2] HEPATITIS-B VIRUS (HBV) X-PROTEIN AND REPLICATION
    KATAYAMA, K
    HAYASHI, N
    SASAKI, Y
    KASAHARA, A
    UEDA, K
    FUSAMOTO, H
    SATO, N
    CHISAKA, O
    MATSUBARA, K
    KAMADA, T
    [J]. HEPATOLOGY, 1988, 8 (05) : 1405 - 1405
  • [3] EXPRESSION OF X-PROTEIN AND HEPATITIS-B VIRUS-REPLICATION IN CHRONIC HEPATITIS
    HARUNA, Y
    HAYASHI, N
    KATAYAMA, K
    YUKI, N
    KASAHARA, A
    SASAKI, Y
    FUSAMOTO, H
    KAMADA, T
    [J]. HEPATOLOGY, 1991, 13 (03) : 417 - 421
  • [4] X-PROTEIN OF HEPATITIS-B VIRUS RESEMBLES A SERINE PROTEASE INHIBITOR
    TAKADA, S
    KOIKE, K
    [J]. JAPANESE JOURNAL OF CANCER RESEARCH, 1990, 81 (12): : 1191 - 1194
  • [5] THE X-PROTEIN OF HEPATITIS-B VIRUS COACTIVATES POTENT ACTIVATION DOMAINS
    HAVIV, I
    VAIZEL, D
    SHAUL, Y
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1995, 15 (02) : 1079 - 1085
  • [6] THE HEPATITIS-B VIRUS X-PROTEIN TARGETS THE BZIP DOMAIN OF CREB
    ANDRISANI, OM
    WILLIAMS, JS
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 27 - 27
  • [7] HEPATITIS-B VIRUS TRANSACTIVATOR X-PROTEIN IS NOT TUMORIGENIC IN TRANSGENIC MICE
    LEE, TH
    FINEGOLD, MJ
    SHEN, RF
    DEMAYO, JL
    WOO, SLC
    BUTEL, JS
    [J]. JOURNAL OF VIROLOGY, 1990, 64 (12) : 5939 - 5947
  • [8] THE EXPRESSION OF X-PROTEIN IN THE LIVER AND HEPATITIS-B VIRUS-REPLICATION IN CHRONIC HEPATITIS
    HARUNA, Y
    HAYASHI, N
    KATAYAMA, K
    KASAHARA, A
    FUSAMOTO, H
    SATO, N
    KAMADA, T
    [J]. HEPATOLOGY, 1990, 12 (02) : 409 - 409
  • [9] MAPPING OF B-CELL EPITOPES OF THE HUMAN HEPATITIS-B VIRUS X-PROTEIN
    STEMLER, M
    WEIMER, T
    TU, ZX
    WAN, DF
    LEVRERO, M
    JUNG, C
    PAPE, GR
    WILL, H
    [J]. JOURNAL OF VIROLOGY, 1990, 64 (06) : 2802 - 2809
  • [10] ACTIVATION OF PROTOONCOGENE C-JUN BY THE X-PROTEIN OF HEPATITIS-B VIRUS
    TWU, JS
    LAI, MY
    CHEN, DS
    ROBINSON, WS
    [J]. VIROLOGY, 1993, 192 (01) : 346 - 350
12345