ANALYSIS OF NUCLEOTIDES, NUCLEOSIDES, NUCLEOBASES IN CELLS BY ION-PAIR REVERSED-PHASE HPLC

被引：17
|
作者：
WERNER, A
机构：
[1] Institute of Biochemistry, Berlin, 1040
来源：
CHROMATOGRAPHIA | 1991年 / 31卷 / 7-8期
关键词：
COLUMN LIQUID CHROMATOGRAPHY; ION-PAIR REVERSED-PHASE SEPARATIONS; NUCLEOTIDES; EXTRACTION FROM CELLS AND TISSUE; RETENTION MECHANISM;
D O I：
10.1007/BF02262200
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
HPLC methods for the separation of nucleotides, nucleosides and nucleobases by ion-pair reversed-phase are reviewed. The advantages of these are discussed versus anion-exchange and reversed phase separations. Extraction procedures for nucleotide determinations from cells and tissues are pointed out in detail. Extracts from red blood cells, Ehrlich ascites tumour cells, hepatocytes, intestine are used for determination of nucleotide concentrations by the methods described.
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页码：401 / 410
页数：10
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