DETECTION OF TICK-BORNE ENCEPHALITIS-VIRUS IN IXODID TICKS COLLECTED IN NATURAL FOCI BY TIME-RESOLVED FLUOROIMMUNOASSAY

被引:0
|
作者
POMELOVA, VG [1 ]
LAVROVA, NA [1 ]
GAIDAMOVICH, SY [1 ]
NAVOLOKIN, OV [1 ]
HALONEN, P [1 ]
SOKOLOVA, MV [1 ]
KHARITONENKOV, IG [1 ]
机构
[1] UNIV TURKU,DEPT VIROL,SF-20520 TURKU 52,FINLAND
关键词
TICK-BORNE ENCEPHALITIS VIRUS; INFECTIVITY OF TICK-VECTOR; TIME-RESOLVED FLUOROIMMUNOASSAY; ENZYME IMMUNOASSAY;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Time-resolved fluoroimmunoassay (TR-FIA) was used for the first time for evaluation of infestation of ixodid ticks with tick-borne encephalitis virus. Comparison of TR-FIA results with those obtained in enzyme immunoassay and by virus isolation confirmed the high efficacy of the method in question. Positive results of TR-FIA coincided with the data of virus isolation in 83.6 % cases, the level of false-negative results did not exceed 1.2 %, the overall time consumption amounted to about 1.2 hr.
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页码:260 / 268
页数:9
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