APPLICATION OF PLURONIC-F68 FOR AQUEOUS 2-PHASE EXTRACTION OF PROTEINS

被引：9
|
作者：
KITAHARA, T [1 ]
KAMIHIRA, M [1 ]
TAKEUCHI, H [1 ]
机构：
[1] NAGOYA UNIV,FAC ENGN,DEPT CHEM ENGN,CHIKUSA KU,NAGOYA 46401,JAPAN
来源：
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 1993年 / 26卷 / 02期
关键词：
BIOCHEMICAL ENGINEERING; AQUEOUS 2-PHASE EXTRACTION; BIOSEPARATION; PLURONIC-F68; BINODAL CURVE; MONOCLONAL ANTIBODY;
D O I：
10.1252/jcej.26.183
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Studies were made of the application of a block copolymer of polyethylene oxide and polypropylene oxide, Pluronic F68, to aqueous two-phase extraction of proteins. For Pluronic F68/salt systems, the effects of temperature, salt concentration and pH on the binodials and protein partitioning were studied. Pluronic F68/salt system had a region in which Pluronic F68 becomes solid in phase diagram at 25-degrees-C. Partitioning of the proteins between the two phases could be controlled by varying salt composition. Further applicability of the Pluronic F68/ammonium sulfate system was examined for the purification of monoclonal antibodies from various hybridoma culture broths. Aqueous two-phase extraction provided a simple and rapid separation and concentration method with high recovery.
引用
收藏
页码：183 / 188
页数：6
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