AMINO-ACID-ANALYSIS OF PROTEIN HYDROLYSATES BY LITHIUM BUFFER SYSTEM

被引:0
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作者
SUWAN, S [1 ]
机构
[1] CHULALONGKORN UNIV,CTR SCI & TECHNOL RES EQUIPMENT,BANGKOK 10330,THAILAND
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暂无
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A chromatographic procedure for the analysis of amino acid hydrolysates using lithium buffers and a dedicated amino acid analyzer is described. When used with an Hitachi amino acid analyzer model 835-50, the modified system permitted separation to near baseline of 18 common amino acids, plus another 6 amino compounds commonly encountered in such assays. Elution time from injection to arginine was 90 minutes. A major advantage of this system when only one amino acid analyzer is available is the greatly reduced work load compared to the classical switching over between sodium-based and lithium-based systems.
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页码:97 / 103
页数:7
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