ELECTROTRANSFORMATION OF STREPTOCOCCUS-PNEUMONIAE

被引：2
|
作者：
CANVIN, JR
ANDREW, PW
BOULNOIS, GJ
MITCHELL, TJ
机构：
[1] UNIV LEICESTER,DEPT MICROBIOL,POB 138,UNIV RD,LEICESTER LE1 9HN,ENGLAND
[2] LEICESTER ROYAL INFIRM,DEPT IMMUNOL,LEICESTER LE2 7LX,ENGLAND
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 01期
关键词：
D O I：
10.1111/j.1472-765X.1994.tb00794.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Conditions of electroporation to transform encapsulated strains of Streptococcus pneumoniae with plasmid DNA have been defined. For a heavily encapsulated strain, an electroporation solution of 10-20% (v/v) glycerol and 3.2 kV cm-1 field strength, 1000 OMEGA resistance and 25 muF capacitance were optimal. For lightly encapsulated or non-encapsulated strains, optimal conditions were a sucrose-based electroporation solution and 12.5 kV cm-1 field strength, 200 OMEGA resistance and 25 muF capacitance.
引用
收藏
页码：35 / 38
页数：4
相关论文
共 50 条
  • [1] STREPTOCOCCUS-PNEUMONIAE SEROTYPING
    GOTTLIEB, T
    [J]. PATHOLOGY, 1989, 21 (02) : 152 - 152
  • [2] PLASMID IN STREPTOCOCCUS-PNEUMONIAE
    SMITH, MD
    GUILD, WR
    [J]. JOURNAL OF BACTERIOLOGY, 1979, 137 (02) : 735 - 739
  • [3] SCLERITIS AND STREPTOCOCCUS-PNEUMONIAE
    ALTMAN, AJ
    COHEN, EJ
    BERGER, ST
    MONDINO, BJ
    [J]. CORNEA, 1991, 10 (04) : 341 - 345
  • [4] ENDOCARDITIS BY STREPTOCOCCUS-PNEUMONIAE
    VALENCIA, ME
    GUINEA, J
    ENRIQUEZ, A
    LAHOZ, JG
    [J]. MEDICINA CLINICA, 1994, 102 (07): : 277 - 278
  • [5] STREPTOCOCCUS-PNEUMONIAE BACTEREMIA
    TORRESTORTOSA, M
    LOPEZ, MD
    SANCHEZPORTO, A
    LOPEZPRIETO, D
    QUINTERO, JC
    [J]. REVISTA CLINICA ESPANOLA, 1993, 193 (09): : 518 - 519
  • [6] STREPTOCOCCUS-PNEUMONIAE ENDOCARDITIS
    SANDS, M
    BROWN, RB
    RYCZAK, M
    HAMILTON, W
    [J]. SOUTHERN MEDICAL JOURNAL, 1987, 80 (06) : 780 - 782
  • [7] BACTERIOPHAGES OF STREPTOCOCCUS-PNEUMONIAE
    LOPEZ, R
    GARCIA, E
    RONDA, C
    [J]. REVIEWS OF INFECTIOUS DISEASES, 1981, 3 (02): : 212 - 223
  • [8] UPTAKE OF CHOLINE BY STREPTOCOCCUS-PNEUMONIAE
    THOMAS, AM
    POXTON, IR
    LAMBERT, PA
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1978, 109 (DEC): : 313 - 317
  • [9] STREPTOCOCCUS-PNEUMONIAE AND EQUINE DISEASE
    CHANTER, N
    [J]. EQUINE VETERINARY JOURNAL, 1994, 26 (01) : 5 - 6
  • [10] RECURRENT STREPTOCOCCUS-PNEUMONIAE ENDOCARDITIS
    CUNNINGHAM, R
    SINHA, L
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1995, 14 (06) : 526 - 528
12345