QUANTIFICATION OF ACUTE PHASE PROTEINS IN RAT SERUM AND IN THE SUPERNATANTS OF A CULTURED RAT HEPATOMA-CELL LINE AND CULTURED PRIMARY HEPATOCYTES BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：16
|
作者：
IKAWA, M
SHOZEN, Y
机构：
[1] Meiji Institute of Health Science, Odawara, Kanagawa
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 134卷 / 01期
关键词：
Acute phase protein; ELISA; Hepatocyte; Hepatoma cell;
D O I：
10.1016/0022-1759(90)90117-E
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme-linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum. Rat APRs of α2-macroglobulin, α1-acid glycoprotein (AGP), haptoglobin (Hp), haemopexin (Hpx), cysteine protease inhibitor (CPI), albumin (Alb) and transferrin (Tf) were purified from the plasma of turpentine treated rats. Fab′ fragments from each IgG fraction were labelled with horseradish peroxidase (POD) by the maleimide method and the assay was performed using a sandwich technique. This ELISA procedure was able to measure nanogram quantities of rat serum APRs. © 1990.
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页码：101 / 106
页数：6
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