EPIDERMAL GROWTH-FACTOR INDUCES SERINE PHOSPHORYLATION OF STATHMIN IN A HUMAN COLON-CARCINOMA CELL-LINE (LIM-1215)

被引：0
|
作者：
JI, H
BALDWIN, GS
BURGESS, AW
MORITZ, RL
WARD, LD
SIMPSON, RJ
机构：
[1] LUDWIG INST CANC RES, JOINT PROT STRUCT LAB, PO ROYAL MELBOURNE HOSP, PARKVILLE, VIC 3050, AUSTRALIA
[2] ROYAL MELBOURNE HOSP, WALTER & ELIZA HALL INST MED RES, PARKVILLE, VIC 3050, AUSTRALIA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1993年 / 268卷 / 18期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Changes in protein phosphorylation in the human colon carcinoma cell line LIM 1215 after stimulation with epidermal growth factor (EGF) have been analyzed by two-dimensional gel electrophoresis and phosphoamino acid analysis. In addition to a number of tyrosine-phosphorylated proteins, a family of small proteins (M(r) 19,000-20,000) is maximally phosphorylated on serine within 5 min of EGF stimulation. One member of the family has been purified by a combination of two-dimensional electrophoresis and reversed-phase high performance liquid chromatography and identified by amino acid sequence analysis as stathmin. Although phosphorylation of stathmin has been reported previously in leukemia cells and following stimulation of hemopoietic or lymphoid cells with several mitogenic agents, this is the first report of stathmin phosphorylation in response to EGF.
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页码：13396 / 13405
页数：10
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