CLONING OF HSP70 (DNAK) GENE FROM CLOSTRIDIUM-PERFRINGENS USING A GENERAL POLYMERASE CHAIN-REACTION BASED APPROACH

被引：33
|
作者：
GALLEY, KA [1 ]
SINGH, B [1 ]
GUPTA, RS [1 ]
机构：
[1] MCMASTER UNIV, DEPT BIOCHEM, HAMILTON L8N 3Z5, ONTARIO, CANADA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 02期
基金：
英国医学研究理事会;
关键词：
HSP70-SEQUENCE; DNAK GENE; POLYMERASE CHAIN REACTION; (CLOSTRIDIUM-PERFRINGENS);
D O I：
10.1016/0167-4781(92)90529-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A general method to clone the HSP70 gene from any species employing polymerasc chain reaction and degenerate oligonucleotide primers for conserved regions of this protein family is described. Using this method, a clone containing the entire coding sequence for the HSP70 gene from Clostridium perfringens, has been isolated and sequenced. The HSP70s from C perfringens as well as other Gram-positive groups of bacteria contain a large deletion of 25 amino acids, near the N-terminal end, which is not seen in HSP70 from other sources.
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页码：203 / 208
页数：6
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