DETERMINATION OF APOVINCAMINIC ACID IN SERUM BY MEANS OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：8
|
作者：
KRAUS, G
SCHULZ, HU
LOHMANN, A
机构：
[1] MED UNIV LUBECK,ANGIOL & GERIATRIE KLIN,FUNKT EINHEIT KLIN PHARMAKOL,W-2400 LUBECK,GERMANY
[2] THIEMANN ARZNEIMITTEL GMBH,REFERAT PHARMAKOKINET,W-4355 WALTROP,GERMANY
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 02期
关键词：
D O I：
10.1016/0378-4347(92)80137-F
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic method for the determination of low concentrations in serum of apovincaminic acid, the main metabolite of vinpocetine, is reported. The assay includes a two-step ion-pair extraction with tetrabutylammonium as counter ion. Recovery is ca. 40%. Separation is performed on a narrow-range 5-mu-m particle size octadecylsilane modified silica packing. Heptanesulphonic acid is the pairing ion in the eluent, and the ultraviolet detection wavelength is 224 nm. Yohimbine serves as the internal standard. The assay is fast, accurate and sensitive quantifying at least 5 ng/ml apovincaminic acid in serum. The method was applied to the analysis of serum samples from aged subjects, treated with a 20-mg dose of vinpocetine.
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页码：323 / 327
页数：5
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