CRYOPRESERVATION OF QUERCUS-FAGINEA EMBRYONIC AXES

被引：20
|
作者：
GONZALEZBENITO, ME
PEREZRUIZ, C
机构：
[1] Departamento de Biologia Vegetal, E.T.S. Ingenieros Agrônomos, Ciudad Universitaria, Madrid
来源：
CRYOBIOLOGY | 1992年 / 29卷 / 06期
关键词：
D O I：
10.1016/0011-2240(92)90072-A
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Embryonic axes, excised or included in cotyledonary tissue, of Quercus faginea were tested for viability after rapid freezing and storage in liquid nitrogen (-196 °C). Explants were previously pretreated by desiccation at different moisture contents or by soaking in cryoprotectant (15% dimethyl sulfoxide). Best germination response after freezing (60%) was observed when embryonic axes were desiccated from 53 to 21% moisture content (on a fresh weight basis). Desiccation below 39% moisture content or freezing produced damage resulting in loss of organized growth and the induction of callus formation. © 1992.
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收藏
页码：685 / 690
页数：6
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