RESPIRATORY SYNCYTIAL VIRUS NUCLEOCAPSID PROTEIN (N) EXPRESSED IN INSECT CELLS FORMS NUCLEOCAPSID-LIKE STRUCTURES

The gene coding for the N protein of RSV strain Long has been cloned and sequenced. It was introduced behind the polyhedrin promoter of the shuttle vector pVL941 and baculoviruses containing the N gene were constructed by homologous recombination. Infection of Spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic N protein. The baculovirus expressed N protein was concentrated in the cytoplasm of the insect cells and could be extracted at low salt concentration. Nucleocapsid structures similar to those purified from RSV-infected cells could be observed by electron microscopy after negative staining of cellular extracts.