HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF CLOMIPRAMINE AND METABOLITES IN HUMAN PLASMA AND URINE

被引：18
|
作者：
NIELSEN, KK
BROSEN, K
机构：
[1] Department of Clinical Pharmacology, Institute of Medical Biology, Odense University, Odense
来源：
THERAPEUTIC DRUG MONITORING | 1993年 / 15卷 / 02期
关键词：
CLOMIPRAMINE; 7; METABOLITES; HUMAN PLASMA AND URINE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET DETECTION;
D O I：
10.1097/00007691-199304000-00010
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
An isocratic, high-performance liquid chromatography method has been developed for simultaneous determination of the tricyclic antidepressant clomipramine and six metabolites: desmethylclomipramine, 2- and 8-hydroxyclomipramine, 2- and 8-hydroxydesmethylclomipramine and didesmethylclomipramine in plasma and urine. In addition, clomipramine N-oxide was determined in the unconjugated fraction of urine. The method is based on a three-step liquid-liquid extraction, the chromatographic eluent was 30% acetonitrile and 70% aqueous sodium perchlorate solution, pH 2.5, and the UV detection was performed at 220 nm. The method was applied to the analysis of samples followed by oral doses of clomipramine to patients and healthy volunteers.
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页码：122 / 128
页数：7
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