PURIFICATION OF HUMAN AND RAT-KIDNEY ALDOSE REDUCTASE

被引：3

作者：

MOECKEL, G ^{[1
]}

HALLBACH, J ^{[1
]}

GUDER, WG ^{[1
]}

机构：

[1] STADT KRANKENHAUS MUNCHEN BOGENHAUSEN,INST KLIN CHEM,D-81925 MUNICH,GERMANY

来源：

ENZYME & PROTEIN | 1994年 / 48卷 / 01期

关键词：

KIDNEY; SORBITOL; POLYOL PATHWAY; ORGANIC OSMOLYTES;

D O I：

10.1159/000474968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study we report on a rapid two-step affinity chromatographic procedure to purify aldose reductase from human and rat kidney papilla and inner medulla. This enzyme, which is responsible for sorbitol formation in the kidney, was purified 145-fold from rat and 76-fold from human kidneys by consecutive Blue Sepharose and Matrex Orange chromatography. SDS-PAGE showed a single band of 38 kD for the human enzyme and a doublet of similar molecular weight for the rat kidney aldose reductase. The enzyme was characterized by substrate specificity and kinetic constants found identical to that of other organs purified previously.

引用

页码：45 / 50

页数：6

共 50 条

[1] RAT-KIDNEY ALDOSE REDUCTASE AND ALDEHYDE REDUCTASE AND POLYOL PRODUCTION IN RAT-KIDNEY
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[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (05): : F799 - F805
[2] ALDOSE REDUCTASE AND SORBITOL DEHYDROGENASE DISTRIBUTION IN RAT-KIDNEY
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[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (01) : 1 - 8
[3] CHARACTERIZATION OF ALDOSE REDUCTASE AND ALDEHYDE REDUCTASE FROM THE MEDULLA OF RAT-KIDNEY
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TANIMOTO, T
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[6] Expression of aldose reductase in developing rat kidney
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[J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2002, 283 (03) : F481 - F491
[7] PURIFICATION AND PROPERTIES OF AN NADPH-LINKED ALDEHYDE REDUCTASE FROM RAT-KIDNEY
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[8] RAPID PURIFICATION OF HUMAN PLACENTAL ALDOSE REDUCTASE
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[9] THE PURIFICATION AND PROPERTIES OF ALDOSE REDUCTASE FROM RAT OVARY
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